ESR9: Localization and interactions of labs-on-a-vesicle with subcellular structures in endothelial pathology models in vitro

at Jagiellonian University Krakow/Poland
Faculty of Chemistry and Jagiellonian Centre for Experimental Therapeutics
Prof. Dr. Malgorzata Baranska (baranska@chemia.uj.edu.pl)

Candidates with a background in chemistry, biochemistry and/or spectroscopy are invited to apply for a PhD position in the Raman Imaging Group (head: Prof. Malgorzata Baranska) in the Faculty of Chemistry at Jagiellonian University (JUK, Krakow, Poland).

The aim of the doctoral project is to elucidate response of supramolecular logic gates (labs-on-a-vesicle) in cellular models of endothelial pathology in order to characterize them in in vitro conditions and evaluate its applicability in in vivo studies. The candidate will determine the distribution of labs-on-a-vesicle in endothelial cells using multimodal (Raman/fluorescence) imaging in vitro in a model of endothelial pathology in order to elucidate their transport, localization and interactions with subcellular structures. As a prerequisite of this step, biochemical markers of a studied endothelial pathology will be determined. To further examine activity of labs-on-a-vesicle, response of the system on light-triggered NO-release molecules will be investigated using the multimodal imaging approach.

Secondments: application of metabolomics methods to validate endothelial cell pathology (2 months at UL/Netherlands), alignment of multimodal spectroscopic data (one month at DCU/Ireland) and response studies in 3D cell models (one month at the company Mimetas/Netherlands).

Requirements: Master/Engineering degree in Chemistry or Biochemistry, preferably with majors in Spectroscopy. Good knowledge and interest in both experimental and theoretical work. Interest in the field of spectroscopy, microscopy and chemometrics applied to biological samples is required. Knowledge in the field of (bio)chemical sensing, endothelial biochemistry are great assets. As an equal opportunity employer Jagiellonian University is committed to increase the percentage of female scientists and therefore especially encourages them to apply.

Planned start date: 1st June 2019

Application deadline: 28th February 2019

Contact: Applications should be sent to Prof. Malgorzata Baranska at baranska@chemia.uj.edu.pl. Please, insert “LogicLab PhD” as the e-mail title.