ESR6: Transient absorption microscopy of a lab-on-a-vesicle in vitro and ex-vivo

at Leibniz-Institute of Photonic Technology Jena/Germany
Department Functional Interfaces
Prof. Dr. Benjamin Dietzek (benjamin.dietzek@leibniz-ipht.de)

Candidates with a background in physics, (bio)chemistry, photonics or optical engineering are invited to apply for a PhD position in the department of Functional Interfaces (head: Prof. Benjamin Dietzek) as part of a European-funded project at Leibniz Institute of Photonic Technology (Leibniz-IPHT Jena, Germany).

The aim of the doctoral project is to study of molecular logic gates and their supramolecular assemblies (lab-on-a-vesicle) in endothelial cells using time-resolved (micro)spectroscopic methods, i.e. transient absorption microscopy. The candidate will investigate spatially resolved photophysics of the molecular sensors and lab-on-a-vesicle in endothelial cells and ex-vivo arteries of small animals and thus elucidate the role of different local environments on the photophysics and the resultant optical properties. During the project he/she will gain first spectroscopic insights into the excited-state processes of isolated and vesicle-integrated molecular chromophores when embedded into a biological environment. The photophysical properties immediately after uptake of the vesicles into the cells / the tissue will be characterised using time-lapse experiments.

Secondments: fluorescence (lifetime) microscopy in cells to correlate the results to the transient absorption microscopic data (2 months secondment at DCU/Ireland), label-free characterisation of artery tissue from atherosclerotic mouse models using Raman-imaging (one month secondment at JUK/Poland), spectroscopic investigation of the (supra)molecular sensors in artery-on-a-chip systems (2 months secondment at the company Mimetas) as well as in endothelial/microglia cell co-cultures (one month secondment at SAS/Slovakia).

Requirements: Master/Engineering degree in Physics, (Bio)Chemistry or Optical Engineering, preferably with majors in Spectroscopy. Good knowledge and interest in both experimental and theoretical work. Interest in the field of photochemistry, photophysics and time-resolved optical spectroscopy is required. Knowledge in the field of (bio)chemical sensing, cell biology are great assets. As an equal opportunity employer Leibniz IPHT is committed to increase the percentage of female scientists and therefore especially encourages them to apply.

Planned start date: 1st July 2019

Application deadline: 30th April 2019

Contact: Applications should be sent to Prof. Benjamin Dietzek at benjamin.dietzek@leibniz-ipht.de

For more information and how to apply click here.